منابع مشابه
Direct bilirubin higher than total bilirubin?
Both total and direct bilirubin measure the formation of azobilirubin, which is detected at 570/660 nm. The total bilirubin reaction contains surfactant and caffeine, which solubilize “indirect” bilirubin and accelerate the reaction (1). Direct bilirubin is a component of total bilirubin and therefore should always be smaller in value. The presence of a monoclonal immunoglobulin (2220mg/dL) fal...
متن کاملThe identification of direct-reacting bilirubin as bilirubin glucuronide.
It has long been recognized that, with p-diazobenzenesulfonic acid (van den Bergh reagent), bilirubin in serum of patients with jaundice may exhibit two types of reaction (1, 2). If coupling with the diazo reagent occurs promptly in aqueous solution, the reaction is said to be “direct;” if, however, coupling t.akes place only after prior addition of alcohol, the reaction is said to be “indirect...
متن کاملMeasurement of direct bilirubin by use of bilirubin oxidase.
We developed an enzymatic method for measuring direct-reacting bilirubin (DBIL) in serum. At pH 4.5, bilirubin oxidase (BOX) oxidizes mono-conjugated bilirubin, di-conjugated bilirubin, and most of the delta-bilirubin to biliverdin. The resulting decrease in absorbance at 460 nm is linearly related to the concentration of DBIL in serum. Mean DBIL values in the 51 patients' sera examined by the ...
متن کاملThe Identification of “direct-reacting” Bilirubin as Bilirubin Glucuronide*
It has long been recognized that, with p-diazobenzenesulfonic acid (van den Bergh reagent), bilirubin in serum of patients with jaundice may exhibit two types of reaction (1, 2). If coupling with the diazo reagent occurs promptly in aqueous solution, the reaction is said to be “direct;” if, however, coupling t.akes place only after prior addition of alcohol, the reaction is said to be “indirect...
متن کاملInaccurate values for direct bilirubin with some commonly used direct bilirubin procedures.
We compared five methods for the determination of total and direct bilirubins in serum samples from normal controls, subjects with Gilbert's syndrome, and serum pools containing about 50 and 150 mg of total bilirubin per liter. The Kodak Ektachem method and a diazotized sulfanilic acid method with 0.15 mmol/L sodium nitrite concentrations are the only methods that gave accurate direct bilirubin...
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ژورنال
عنوان ژورنال: Okayama Igakkai Zasshi (Journal of Okayama Medical Association)
سال: 1972
ISSN: 0030-1558,1882-4528
DOI: 10.4044/joma1947.84.9-10_331